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Image Search Results
Journal: STAR protocols
Article Title: A high-throughput protocol for monitoring starvation-induced autophagy in real time in mouse embryonic fibroblasts.
doi: 10.1016/j.xpro.2021.100966
Figure Lengend Snippet: Figure 4. Comparison between turboGFP-LC3B and eGFP-LC3B MEFs were infected with plasmids expressing either turboGFP-LC3B (left) or eGFP-LC3B (right) and imaged using the IncuCyte. Representative images are shown. Scale bars are 300 mm.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies LC3B Cell signalling TECHNOLOGY Cat#4108 RRID:AB_2137703 b-actin Sigma-Aldrich Cat#A2228 RRID:AB_476697 Chemicals, peptides, and recombinant proteins Polybrene (Hexadimethrine bromide) Sigma-Aldrich Cat#H9268 Experimental models: Cell lines Phoenix-ECO cells ATCC Cat#CRL-3214 RRID:CVCL_H717 Phoenix-AMPHO cells ATCC Cat#CRL-3213 RRID:CVCL_H716 Mouse embryo fibroblasts N/A N/A Recombinant DNA pQCXIP turboGFP LC3 Nowosad et al., (2021) N/A pQCXIP turboGFP p62 Nowosad et al., (2021) N/A Software and algorithms IncuCyte ZOOM 2018A Essen BioScience N/A Microsoft Excel 2010 Microsoft N/A GraphPad Prism 8.0 Prism N/A Other DMEM w/o glucose cell medium Sigma-Aldrich Cat#D5030 DMEM w/o amino acids cell medium USBiological life sciences Cat#D9800-13 DMEM high glucose medium Sigma-Aldrich Cat#D6429, Earle’s Balanced Salt Solution (EBSS) Sigma-Aldrich Cat#E7510
Techniques: Comparison, Infection, Expressing